Open AccessDetection of swelling of single isolated mitochondrion with optical microscopy
Author(s) -
Daisuke Morikawa,
Keita Kanematsu,
Takahiro Shibata,
Keisuke Haseda,
Norihiro Umeda,
Yoshihiro Ohta
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.000848
Subject(s) - organelle , mitochondrion , tonicity , swelling , microscopy , biophysics , volume (thermodynamics) , chemistry , microbiology and biotechnology , materials science , biology , optics , biochemistry , physics , quantum mechanics , composite material
Volume regulation under osmotic loading is one of the most fundamental functions in cells and organelles. However, the effective method to detect volume changes of a single organelle has not been developed. Here, we present a novel technique for detecting volume changes of a single isolated mitochondrion in aqueous solution based on the transmittance of the light through the mitochondrion. We found that 70% and 21% of mitochondria swelled upon addition of a hypotonic solution and Ca(2+), respectively. These results show the potential of the present technique to detect the physiological volume changes of individual small organelles such as mitochondria.