Open Access4D tracking of clinical seminal samples for quantitative characterization of motility parameters
Author(s) -
Giuseppe Di Caprio,
Ahmed El Mallahi,
Pietro Ferraro,
Roberta Dale,
G. Coppola,
Brian Dale,
Giuseppe Coppola,
Frank Dubois
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.000690
Subject(s) - digital holography , motility , digital holographic microscopy , sperm motility , microscopy , semen , sperm , tracking (education) , focus (optics) , semen analysis , characterization (materials science) , optics , computer science , holography , computer vision , artificial intelligence , materials science , biology , physics , microbiology and biotechnology , anatomy , infertility , psychology , pregnancy , pedagogy , botany , genetics
In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.