Open AccessAutomatic segmentation of up to ten layer boundaries in SD-OCT images of the mouse retina with and without missing layers due to pathology
Author(s) -
Pratul P. Srinivasan,
Stephanie J. Heflin,
Joseph A. Izatt,
Vadim Y. Arshavsky,
Sina Farsiu
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.000348
Subject(s) - optical coherence tomography , segmentation , retinal , computer science , retina , artificial intelligence , computer vision , image segmentation , pattern recognition (psychology) , image processing , ophthalmology , medicine , biology , image (mathematics) , neuroscience
Accurate quantification of retinal layer thicknesses in mice as seen on optical coherence tomography (OCT) is crucial for the study of numerous ocular and neurological diseases. However, manual segmentation is time-consuming and subjective. Previous attempts to automate this process were limited to high-quality scans from mice with no missing layers or visible pathology. This paper presents an automatic approach for segmenting retinal layers in spectral domain OCT images using sparsity based denoising, support vector machines, graph theory, and dynamic programming (S-GTDP). Results show that this method accurately segments all present retinal layer boundaries, which can range from seven to ten, in wild-type and rhodopsin knockout mice as compared to manual segmentation and has a more accurate performance as compared to the commercial automated Diver segmentation software.