Open AccessNon-de-scanned parallel recording two-photon hyperspectral microscopy with high spectral and spatial resolution
Author(s) -
Szu Yu Chen,
Chiao Sheng Lu,
Chia Hua Yeh
Publication year - 2014
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.000338
Subject(s) - hyperspectral imaging , optics , microscopy , image resolution , microscope , two photon excitation microscopy , crosstalk , resolution (logic) , dwell time , optical sectioning , distortion (music) , materials science , photon counting , physics , photon , computer science , optoelectronics , artificial intelligence , medicine , clinical psychology , amplifier , cmos , fluorescence
A two-photon hyperspectral microscope with non-de-scanned geometry and parallel recording scheme was constructed which had a low dwell time, high spectral resolution, and high spatial resolution. The dwell time was effectively decreased to reduce photodamage through parallel recording, while the non-de-scanned geometry led to a considerable reduction in the signal loss and spectrum distortion. Two experiments were performed to show that this system can solve crosstalk issues and spectrally resolve the intrinsic fluorophores in optically-thick tissues without staining and sectioning.