Open AccessDual-band Fourier domain optical coherence tomography with depth-related compensations
Author(s) -
Miao Zhang,
Lixin Ma,
Ping Yu
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.000167
Subject(s) - optical coherence tomography , optics , attenuation , speckle pattern , materials science , contrast (vision) , multi band device , tomography , optical tomography , fourier transform , diffuse optical imaging , biological imaging , coherence (philosophical gambling strategy) , biomedical engineering , physics , computer science , medicine , telecommunications , quantum mechanics , antenna (radio) , fluorescence
Dual-band Fourier domain optical coherence tomography (FD-OCT) provides depth-resolved spectroscopic imaging that enhances tissue contrast and reduces image speckle. However, previous dual-band FD-OCT systems could not correctly give the tissue spectroscopic contrast due to depth-related discrepancy in the imaging method and attenuation in biological tissue samples. We designed a new dual-band full-range FD-OCT imaging system and developed an algorithm to compensate depth-related fall-off and light attenuation. In our imaging system, the images from two wavelength bands were intrinsically overlapped and their intensities were balanced. The processing time of dual-band OCT image reconstruction and depth-related compensations were minimized by using multiple threads that execute in parallel. Using the newly developed system, we studied tissue phantoms and human cancer xenografts and muscle tissues dissected from severely compromised immune deficient mice. Improved spectroscopic contrast and sensitivity were achieved, benefiting from the depth-related compensations.