Open AccessCharacterization of a synthetic bioactive polymer by nonlinear optical microscopy
Author(s) -
Nadia Djaker,
Sophie Brustlein,
Géraldine Rohman,
Stéphane Huot,
Marc Lamy de la Chapelle,
Véronique Migonney
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.5.000149
Subject(s) - biocompatibility , materials science , tissue engineering , protein adsorption , polymer , polyester , autofluorescence , adhesion , nanotechnology , grafting , cell adhesion , biomaterial , microscopy , surface modification , biomedical engineering , chemical engineering , composite material , fluorescence , optics , medicine , physics , engineering , metallurgy
Tissue Engineering is a new emerging field that offers many possibilities to produce three-dimensional and functional tissues like ligaments or scaffolds. The biocompatibility of these materials is crucial in tissue engineering, since they should be integrated in situ and should induce a good cell adhesion and proliferation. One of the most promising materials used for tissue engineering are polyesters such as Poly-ε-caprolactone (PCL), which is used in this work. In our case, the bio-integration is reached by grafting a bioactive polymer (pNaSS) on a PCL surface. Using nonlinear microscopy, PCL structure is visualized by SHG and proteins and cells by two-photon excitation autofluorescence generation. A comparative study between grafted and nongrafted polymer films is provided. We demonstrate that the polymer grafting improves the protein adsorption by a factor of 75% and increase the cell spreading onto the polymer surface. Since the spreading is directly related to cell adhesion and proliferation, we demonstrate that the pNaSS grafting promotes PCL biocompatibility.