Open AccessOptical biosensor based on SERS with signal calibration function for quantitative detection of carcinoembryonic antigen
Author(s) -
Tingyin Wang,
Youzhi Zhu,
Shuyun Weng,
Xueliang Lin,
KienVoon Kong,
Youliang Weng,
Xiang gang,
Rong Chen,
Duo Lin,
Feng Yuan
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.474273
Subject(s) - carcinoembryonic antigen , detection limit , materials science , raman scattering , aptamer , colloidal gold , calibration , biosensor , nanotechnology , calibration curve , raman spectroscopy , biomedical engineering , nanoparticle , optics , cancer , chemistry , chromatography , medicine , physics , quantum mechanics , biology , genetics
Monitoring the levels of cancer biomarkers is essential for cancer diagnosis and evaluation. In this study, a novel sandwich type sensing platform based on surface-enhanced Raman scattering (SERS) technology was developed for the detection of carcinoembryonic antigen (CEA), with a limit of detection (LOD) of 0.258 ng/mL. In order to achieve sensitive detection of CEA in complex samples, gold nanoparticle monolayer modified with CEA antibodies and with aptamer-functionalized probes was fabricated to target CEA. Two gold layers were integrated into the SERS platform, which greatly enhanced the signal of the probe by generating tremendous "hot spots". Meanwhile, the intensity ratio of Raman probes and the second-order peak of the silicon wafer was used to achieve dynamic calibration of the Raman probe signal. Excitingly, this sensing platform was capable of distinguishing cancer patients from healthy individuals via CEA concentrations in blood samples with the accuracy of 100%. This sandwich structure SERS sensing platform presented promising potential to be an alternative tool for clinical biomarker detection in the field of cancer diagnosis.