Open AccessThree-dimensional mechanical characterization of murine skeletal muscle using quantitative micro-elastography
Author(s) -
Erin M. Lloyd,
Matt S. Hepburn,
Jiayue Li,
Alireza Mowla,
Yongsung Hwang,
Yu Suk Choi,
Miranda D. Grounds,
Brendan F. Kennedy
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.471062
Subject(s) - dysferlin , skeletal muscle , elastography , muscular dystrophy , elasticity (physics) , myosin , biomedical engineering , anatomy , pathology , chemistry , biology , medicine , materials science , ultrasound , biophysics , radiology , composite material
Skeletal muscle function is governed by both the mechanical and structural properties of its constituent tissues, which are both modified by disease. Characterizing the mechanical properties of skeletal muscle tissue at an intermediate scale, i.e. , between that of cells and organs, can provide insight into diseases such as muscular dystrophies. In this study, we use quantitative micro-elastography (QME) to characterize the micro-scale elasticity of ex vivo murine skeletal muscle in three-dimensions in whole muscles. To address the challenge of achieving high QME image quality with samples featuring uneven surfaces and geometry, we encapsulate the muscles in transparent hydrogels with flat surfaces. Using this method, we study aging and disease in quadriceps tissue by comparing normal wild-type (C57BL/6J) mice with dysferlin-deficient BLAJ mice, a model for the muscular dystrophy dysferlinopathy, at 3, 10, and 24 months of age (sample size of three per group). We observe a 77% decrease in elasticity at 24 months in dysferlin-deficient quadriceps compared to wild-type quadriceps.