Open AccessQuantitative tumor depth determination using dual wavelength excitation fluorescence
Author(s) -
C. O’Brien,
Kevin W. Bishop,
Haini Zhang,
Xin Xu,
Leonid Shmuylovich,
Elizabeth Conley,
Karen Nwosu,
Katie Duncan,
Suman B. Mondal,
Gail Sudlow,
Samuel Achilefu
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.468059
Subject(s) - fluorophore , fluorescence , excitation wavelength , materials science , wavelength , optics , penetration depth , near infrared spectroscopy , fluorescence lifetime imaging microscopy , excitation , attenuation , biomedical engineering , optoelectronics , medicine , physics , quantum mechanics
Quantifying solid tumor margins with fluorescence-guided surgery approaches is a challenge, particularly when using near infrared (NIR) wavelengths due to increased penetration depths. An NIR dual wavelength excitation fluorescence (DWEF) approach was developed that capitalizes on the wavelength-dependent attenuation of light in tissue to determine fluorophore depth. A portable dual wavelength excitation fluorescence imaging system was built and tested in parallel with an NIR tumor-targeting fluorophore in tissue mimicking phantoms, chicken tissue, and in vivo mouse models of breast cancer. The system showed high accuracy in all experiments. The low cost and simplicity of this approach make it ideal for clinical use.