Open AccessIncreasing the field-of-view in oblique plane microscopy via optical tiling
Author(s) -
Bingying Chen,
Bo-Jui Chang,
Felix Zhou,
Stephan Daetwyler,
Etai Sapoznik,
Benjamin A. Nanes,
Isabella Terrazas,
Gabriel M. Gihana,
Lizbeth Perez Castro,
Isaac S. Chan,
Maralice Connacci Sorrell,
Kevin M. Dean,
Alfred Millett-Sikking,
Andrew G. York,
Reto Fiolka
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.467969
Subject(s) - optics , image resolution , microscopy , light sheet fluorescence microscopy , microscope , resolution (logic) , field of view , frame rate , magnification , cardinal point , pixel , computer vision , computer science , materials science , artificial intelligence , physics , scanning confocal electron microscopy
Fast volumetric imaging of large fluorescent samples with high-resolution is required for many biological applications. Oblique plane microscopy (OPM) provides high spatiotemporal resolution, but the field of view is typically limited by its optical train and the pixel number of the camera. Mechanically scanning the sample or decreasing the overall magnification of the imaging system can partially address this challenge, albeit by reducing the volumetric imaging speed or spatial resolution, respectively. Here, we introduce a novel dual-axis scan unit for OPM that facilitates rapid and high-resolution volumetric imaging throughout a volume of 800 × 500 × 200 microns. This enables us to perform volumetric imaging of cell monolayers, spheroids and zebrafish embryos with subcellular resolution. Furthermore, we combined this microscope with a multi-perspective projection imaging technique that increases the volumetric interrogation rate to more than 10 Hz. This allows us to rapidly probe a large field of view in a dimensionality reduced format, identify features of interest, and volumetrically image these regions with high spatiotemporal resolution.