Open AccessDual-wavelength multimodal multiphoton microscope with SMA-based depth scanning
Author(s) -
Wentao Wu,
Qihao Liu,
Christoph Brandt,
Shuo Tang
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.456390
Subject(s) - materials science , optics , femtosecond , two photon excitation microscopy , second harmonic generation , microscopy , sma* , microscope , laser , optoelectronics , wavelength , scanning electron microscope , fluorescence , physics , computer science , algorithm
We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. The multimodal capability is realized by an Er-doped femtosecond fiber laser with dual output wavelengths of 1580 nm and 790 nm that are responsible for three-photon and two-photon excitation, respectively. A shape-memory-alloy (SMA) actuated miniaturized objective enables the depth scanning capability. Image stacks combined with two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG) signals have been acquired from animal, fungus, and plant tissue samples with a maximum depth range over 200 µm.