Open AccessBessel-beam illumination Raman microscopy
Author(s) -
Kiyoshi Bando,
Shumpei Yabuuchi,
Menglu Li,
Takanori Kubo,
Ryosuke Oketani,
Nicholas I. Smith,
Katsumasa Fujita
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.456138
Subject(s) - bessel beam , optics , microscopy , raman spectroscopy , hyperspectral imaging , materials science , bessel function , contrast (vision) , live cell imaging , microscope , beam (structure) , physics , chemistry , cell , computer science , biochemistry , artificial intelligence
We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.