Bessel-beam illumination Raman microscopy | Zendy

Kazuki Bando | Zendy; Shumpei Yabuuchi | Zendy; Menglu Li | Zendy; Toshiki Kubo | Zendy; Ryosuke Oketani | Zendy; Nicholas I. Smith | Zendy; Katsumasa Fujita | Zendy

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Bessel-beam illumination Raman microscopy

Author(s) -

Kazuki Bando,

Shumpei Yabuuchi,

Menglu Li,

Toshiki Kubo,

Ryosuke Oketani,

Nicholas I. Smith,

Katsumasa Fujita

Publication year - 2022

Publication title -

biomedical optics express

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.456138

Subject(s) - bessel beam , optics , microscopy , hyperspectral imaging , raman spectroscopy , bessel function , materials science , contrast (vision) , live cell imaging , microscope , physics , chemistry , computer science , artificial intelligence , cell , biochemistry

We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

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