Open AccessMRI/fluorescence dual-mode probe: its simple preparation method and imaging application in vitro
Author(s) -
Qiang Zhang,
Zhiyong Wu,
Jianqiao Song,
Boye Zhang,
Qianqian Duan,
Darui Song,
Linjun Hu,
Sijin Li,
Shengbo Sang
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.455679
Subject(s) - materials science , superparamagnetism , fourier transform infrared spectroscopy , fluorescence , biocompatibility , fluorescence lifetime imaging microscopy , composite number , mri contrast agent , nuclear magnetic resonance , x ray photoelectron spectroscopy , biomedical engineering , nanotechnology , nanoparticle , analytical chemistry (journal) , chemical engineering , optics , composite material , chemistry , magnetization , magnetic field , organic chemistry , physics , medicine , quantum mechanics , engineering , metallurgy
Superparamagnetic nanoparticles have been widely used as contrast agents in magnetic resonance imaging (MRI). The combined use of multiple imaging modes can provide more accurate information for clinical diagnosis. In this paper, a MRI/fluorescence dual-mode imaging contrast agent was developed by a simple method. The method is to make the fluorescent carbon quantum dots (CDs) adsorbed on the surface of the magnetic composite with pore structure by ultrasonic dispersion. Replacing the traditional methods such as chemical bonding, the fluorescent material is coated on the surface of the composite material. The synthesized composite materials were characterized by the transmission electron microscopy method (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and vibration sample magnetometer (VSM). The results of TEM, FTIR and XPS showed that CDs were successfully coated on the surface of C 60 @Fe 3 O 4 magnetic composite. The VSM results show that the composite material still maintains superparamagnetism. The cytotoxicity of the material on SMMC-7721 liver cancer cells was detected by the MTT method, and the biocompatibility of the material was verified. By observing the fluorescence distribution in the cell, it is proved that the composite material successfully enters the cell and produces fluorescence. Finally, through the analysis of T 2 -weighted imaging, it is found that the addition of materials results in an enhanced dark contrast compared to control cells. Therefore, the composite nanomaterials synthesized in this paper can be used as MRI/fluorescence dual-mode imaging contrast agents.