Open AccessOptimization of photo-biomodulation therapy for wound healing of diabetic foot ulcers in vitro and in vivo
Author(s) -
Qianqian Chen,
Jichun Yang,
Huijuan Yin,
Yingxin Li,
Haixia Qiu,
Yajia Gu,
Hua Yang,
Xiaoxi Dong,
Xiafei Shi,
Bochen CHE,
Hongxiao LI
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.451135
Subject(s) - in vivo , wound healing , diabetic foot , angiogenesis , diabetic foot ulcer , medicine , in vitro , inflammation , diabetic ulcers , diabetes mellitus , cancer research , surgery , chemistry , biology , endocrinology , biochemistry , microbiology and biotechnology
Unclear optical parameters make photo-biomodulation (PBM) difficult to implement in diabetic foot ulcer (DFU) clinically. Here, 12 wavelengths (400-900 nm) were used to conduct PBM to heal DFU wounds in vitro and in vivo. PBM at 10 mW/cm 2 and 0.5-4 J/cm 2 with all 12 wavelengths promoted proliferation of diabetic wound cells. In a mimic DFU (mDFU) rat model, PBM (425, 630, 730, and 850 nm, and a combination light strategy) promoted mDFU healing. The positive cell proliferation, re-epithelialization, angiogenesis, collagen synthesis, and inflammation were possible mechanisms. The combination strategy had the best effect, which can be applied clinically.