Open AccessGastric cancer multicellular spheroid analysis by two-photon microscopy
Author(s) -
George Alzeeb,
Matthieu Dubreuil,
Danielle Arzur,
Sylvain Rivet,
Laurent Corcos,
Yann Le Grand,
Catherine CORCOS
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.450518
Subject(s) - spheroid , cancer cell , cancer , 3d cell culture , multicellular organism , cell culture , cellular pathology , microscopy , cell , tumor microenvironment , biology , chemistry , cancer research , pathology , microbiology and biotechnology , medicine , genetics
Gastric cancer (GC) is highly deadly. Three-dimensional (3D) cancer cell cultures, known as spheroids, better mimic tumor microenvironment (TME) than standard 2D cultures. Cancer-associated fibroblasts (CAF), a major cellular component of TME, promote or restrain cancer cell proliferation, invasion and resistance to drugs. We established spheroids from two human GC cell lines mixed with human primary CAF. Spheroid organization, analyzed by two-photon microscopy, showed CAF in AGS/CAF spheroids clustered in the center, but dispersed throughout in HGT-1/CAF spheroids. Such differences may reflect clonal specificities of GC cell lines and point to the fact that GC should be considered as a highly personalized disease.