Open AccessDiamond Raman laser and Yb fiber amplifier for in vivo multiphoton fluorescence microscopy
Author(s) -
Shaun Engelmann,
Annie Zhou,
Ahmed M. Hassan,
Michael Williamson,
Jeremy W. Jarrett,
Evan P. Perillo,
Alankrit Tomar,
David J. Spence,
Theresa A. Jones,
Andrew K. Dunn
Publication year - 2022
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.448978
Subject(s) - materials science , laser , optics , raman spectroscopy , amplifier , microscopy , two photon excitation microscopy , raman scattering , fluorescence , fiber laser , excitation , fluorescence microscope , optoelectronics , optical fiber , fluorescence lifetime imaging microscopy , diamond , wavelength , physics , cmos , quantum mechanics , composite material
Here we introduce a fiber amplifier and a diamond Raman laser that output high powers (6.5 W, 1.3 W) at valuable wavelengths (1060 nm, 1250 nm) for two-photon excitation of red-shifted fluorophores. These custom excitation sources are both simple to construct and cost-efficient in comparison to similar custom and commercial alternatives. Furthermore, they operate at a repetition rate (80 MHz) that allows fast image acquisition using resonant scanners. With our system we demonstrate compatibility with fast resonant scanning, the ability to acquire neuronal images, and the capability to image vasculature at deep locations (>1 mm) within the mouse cerebral cortex.