Open AccessThree-dimensional analysis of blood platelet spreading using digital holographic microscopy: a statistical study of the differential effect of coatings in healthy volunteers and dialyzed patients
Author(s) -
Jérôme Dohet-Eraly,
Karim Zouaoui Boudjeltia,
Alexandre Fontaine Rousseau,
Patrick Queeckers,
Christophe Lelubre,
Jean-Marc Desmet,
Bastien Chopard,
Catherine Yourassowsky,
Frank Dubois
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.448817
Subject(s) - platelet , digital holographic microscopy , hemostasis , holography , microscopy , medicine , biomedical engineering , optics , pathology , physics
In cardiovascular disorders, the study of thrombocytes, commonly known as platelets, is highly important since they are involved in blood clotting, essential in hemostasis, and they can in pathological situations affect the blood circulation. In this paper, single deposited platelets are measured using interferometric digital holographic microscopy. We have shown that the average optical height of platelets is significantly lower in healthy volunteers than in dialyzed patients, meaning a better spreading. It demonstrates the great interest for assessing this parameter in any patients, and therefore the high potential of analyzing single spread platelets using digital holographic microscopy in fundamental research as well as a diagnostic tool in routine laboratories, for usual blood tests.