Open AccessCytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery
Author(s) -
Sandra Pampín-Suárez,
J. L. Arce-Diego,
Olga Tapia,
Flor M. Pérez-Campo,
José C. RodríguezRey,
F. Fanjul-Vélez
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.447773
Subject(s) - cytotoxicity , confocal microscopy , immunofluorescence , biomedical engineering , confocal , materials science , fluorescence microscope , biomarker , collateral damage , fluorescence lifetime imaging microscopy , pathology , fluorescence , medicine , chemistry , optics , microbiology and biotechnology , in vitro , biology , antibody , immunology , sociology , biochemistry , physics , criminology
Biological tissue discrimination is relevant in guided surgery. Nerve identification is critical to avoid potentially severe collateral damage. Fluorescence imaging by oxazine 4-perchlorate (O4P) has been recently proposed. In this work, the cytotoxicity of O4P on U87 human-derived glioma cells has been investigated as a function of concentration and operating room irradiation modes. A custom-built optical irradiation device was employed for controlled optical dosimetry. DNA damage and O4P intracellular localization was also investigated by immunofluorescence and confocal microscopy. The results show that concentration below 100 µM can be considered safe. These results contribute to the assessment of the feasibility of O4P as a nerve biomarker.