Open AccessThree-dimensional dynamics optical coherence tomography for tumor spheroid evaluation
Author(s) -
Ibrahim Abd El-Sadek,
Akiyoshi Miyazawa,
Larina TzuWei Shen,
Shuichi Makita,
Pradipta Mukherjee,
Antonia Lichtenegger,
Satoshi Matsusaka,
Yoshiaki Yasuno
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.440444
Subject(s) - optical coherence tomography , spheroid , tomography , dynamics (music) , biomedical engineering , microscopy , diffuse optical imaging , optics , computer science , physics , chemistry , medicine , biochemistry , acoustics , in vitro
We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.