Open AccessMultimodal hyperspectral fluorescence and spatial frequency domain imaging for tissue health diagnostics of the oral cavity
Author(s) -
Ben E. Urban,
Hrebesh M. Subhash
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.439663
Subject(s) - hyperspectral imaging , artificial intelligence , computer science , computer vision , medical imaging , optics , biomedical engineering , medicine , physics
A multimodal, hyperspectral imaging system was built for diagnostics of oral tissues. The system, termed Hy perspectral- F luorescence- S patial F requency D omain I maging (Hy-F-SFDI), combines the principles of spatial frequency domain imaging, quantitative light fluorescence, and CIELAB color measurement. Hy-F-SFDI employs a compact LED projector, excitation LED, and a 16 channel hyperspectral camera mounted on a custom platform for tissue imaging. A two layer Monte Carlo approach was used to generate a reference table for quick tissue analysis. To demonstrate the clinical capabilities of Hy-F-SFDI, we used the system to quantify gingival tissue hemoglobin volume fraction, detect caries, bacterial activity, and measure tooth color of a volunteer at different time points. Hy-F-SFDI was able to measure quantitative changes in tissue parameters.