Open AccessQuantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy
Author(s) -
Soongho Park,
Thien Nguyen,
Emilie Benoit,
Dan L. Sackett,
Marcial GarmendiaCedillos,
Randall Pursley,
Claude Boccara,
Amir Gandjbakhche
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.436330
Subject(s) - hela , viability assay , microscopy , coherence (philosophical gambling strategy) , biophysics , optical coherence tomography , intracellular , optical microscope , optics , materials science , cell , chemistry , microbiology and biotechnology , biology , physics , biochemistry , scanning electron microscope , quantum mechanics
Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.