Open AccessHigh-speed multifocus phase imaging in thick tissue
Author(s) -
Sheng Xiao,
Shuqi Zheng,
Jérôme Mertz
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.436247
Subject(s) - optics , biological imaging , microscopy , phase imaging , phase (matter) , depth of field , refraction , light sheet fluorescence microscopy , materials science , refractive index , phase contrast imaging , prism , splitter , phase contrast microscopy , physics , scanning confocal electron microscopy , quantum mechanics , fluorescence
Phase microscopy is widely used to image unstained biological samples. However, most phase imaging techniques require transmission geometries, making them unsuited for thick sample applications. Moreover, when applied to volumetric imaging, phase imaging generally requires large numbers of measurements, often making it too slow to capture live biological processes with fast 3D index-of-refraction variations. By combining oblique back-illumination microscopy and a z-splitter prism, we perform phase imaging that is both epi-mode and multifocus, enabling high-speed 3D phase imaging in thick, scattering tissues with a single camera. We demonstrate here 3D qualitative phase imaging of blood flow in chick embryos over a field of view of 546 × 546 × 137 µm 3 at speeds up to 47 Hz.