Open AccessAnalysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
Author(s) -
Matteo Bernardello,
Radoslaw J Gora,
Patrick van Hage,
Gustavo Castro-Olvera,
Emilio J. Gualda,
Marcel J. M. Schaaf,
Pablo Loza-Álvarez
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.435103
Subject(s) - light sheet fluorescence microscopy , microscopy , zebrafish , fluorescence microscope , live cell imaging , biophysics , intracellular , fluorescence , microbiology and biotechnology , photoactivated localization microscopy , biological system , nanotechnology , super resolution microscopy , biology , materials science , optics , cell , physics , biochemistry , gene
Single-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet fluorescence microscopy setup is presented for imaging individual proteins inside living zebrafish embryos. The optical configuration makes this design accessible to many laboratories and a dedicated sample-mounting system ensures sample viability and mounting flexibility. Using this setup, we have analyzed the dynamics of individual glucocorticoid receptors, which demonstrates that this approach creates multiple possibilities for the analysis of intracellular protein dynamics in intact living organisms.