Open AccessOpto-chemical treatment for enhanced high-level disinfection of mature bacterial biofilm in a Teflon-based endoscope model
Author(s) -
Vân Nam Tran,
Periaswamy Sivagnanam Saravana,
Suhyun Park,
Van Gia Truong,
ByungSoo Chun,
Hyun Wook Kang
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.434047
Subject(s) - biofilm , disinfectant , endoscope , staphylococcus aureus , antimicrobial , photosensitizer , microbiology and biotechnology , materials science , chemistry , nanotechnology , medicine , bacteria , surgery , photochemistry , biology , genetics , organic chemistry
Medical societies and public health agencies rigorously emphasize the importance of adequate disinfection of flexible endoscopes. The aim of this work was to propose a novel opto-chemical disinfection treatment against Staphylococcus aureus grown in mature biofilm on Teflon-based endoscope channel models. Laser irradiation using near-infrared and blue wavelengths combined with a low concentration of chemical disinfectant induced both irreversible thermal denaturation and intercellular oxidative stress as a combined mechanism for an augmented antimicrobial effect. The opto-chemical method yielded a 6.7-log 10 reduction of the mature Staphylococcus aureus biofilms (i.e., approximately 1.0-log 10 higher than current requirement of standard treatment). The proposed technique may be a feasible disinfection method for mitigating the risk associated with infection transmission.