Open AccessRobust functional imaging of taste sensation with a Bessel beam
Author(s) -
Jisoo Han,
Seonghoon Kim,
Pyonggang Choi,
Sungho Lee,
Yongjae Jo,
Eun-Soo Kim,
Myunghwan Choi
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.430643
Subject(s) - bessel beam , optics , refractive index , stimulus (psychology) , materials science , physics , beam (structure) , psychology , psychotherapist
Functional imaging of intact taste cells in response to various tastant solutions poses a technical challenge since the refractive index of the immersion medium dynamically changes during tastant delivery. Critically, the focal shift introduced by high-index tastant solutions has been the fundamental limit in experimental design. Here we seek to address this issue by introducing an axially elongated Bessel beam in two-photon microscopy. Compared to the conventional Gaussian beam, the Bessel beam provides superior robustness to the index-induced focal shift, allowing us to acquire near artifact-free imaging of taste cells in response to a physiological taste stimulus.