Open AccessFemtosecond diode-based time lens laser for multiphoton microscopy
Author(s) -
Y. Lange Simmons,
Kenneth J. Underwood,
Omkar D. Supekar,
Brendan M. Heffernan,
T. Welton,
Emily A. Gibson,
Juliet T. Gopinath
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.430549
Subject(s) - femtosecond , laser , optics , materials science , microscopy , microscope , lens (geology) , optoelectronics , diode , x ray laser , light sheet fluorescence microscopy , two photon excitation microscopy , far infrared laser , laser diode , laser power scaling , wavelength , scanning confocal electron microscopy , fluorescence , physics
We demonstrate a near-infrared, femtosecond, diode laser-based source with kW peak power for two-photon microscopy. At a wavelength of 976 nm, the system produces sub-ps pulses operating at a repetition rate of 10 MHz with kilowatt class peak powers suitable for deep tissue two-photon microscopy. The system, integrated with a laser-scanning microscope, images to a depth of 900 µm in a fixed sample of PLP-eGFP labeled mouse brain tissue. This represents a significant development that will lead to more efficient, compact, and accessible laser sources for biomedical imaging.