Open AccessClosed-loop wavefront sensing and correction in the mouse brain with computed optical coherence microscopy
Author(s) -
Siyang Liu,
Fei Xia,
Xusan Yang,
Meiqi Wu,
Laurie A. Bizimana,
Chris Xu,
Steven G. Adie
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.427979
Subject(s) - optics , wavefront , optical coherence tomography , adaptive optics , coma (optics) , coherence (philosophical gambling strategy) , microscopy , physics , signal (programming language) , confocal microscopy , wavefront sensor , interferometry , computer science , quantum mechanics , programming language
Optical coherence microscopy (OCM) uses interferometric detection to capture the complex optical field with high sensitivity, which enables computational wavefront retrieval using back-scattered light from the sample. Compared to a conventional wavefront sensor, aberration sensing with OCM via computational adaptive optics (CAO) leverages coherence and confocal gating to obtain signals from the focus with less cross-talk from other depths or transverse locations within the field-of-view. Here, we present an investigation of the performance of CAO-based aberration sensing in simulation, bead phantoms, and ex vivo mouse brain tissue. We demonstrate that, due to the influence of the double-pass confocal OCM imaging geometry on the shape of computed pupil functions, computational sensing of high-order aberrations can suffer from signal attenuation in certain spatial-frequency bands and shape similarity with lower order counterparts. However, by sensing and correcting only low-order aberrations (astigmatism, coma, and trefoil), we still successfully corrected tissue-induced aberrations, leading to 3× increase in OCM signal intensity at a depth of ∼0.9 mm in a freshly dissected ex vivo mouse brain.