Open AccessLabel-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique
Author(s) -
Rajesh Desapogu,
Gilles Marchand,
Rebecca Smith,
Paulami Ray,
Émilie Gillier,
Stéphanie Dutertre,
Mehdi Alouini,
Marc Tramier,
Sébastien Huet,
Julien Fade
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.426630
Subject(s) - microscopy , optics , polarimetry , microscope , confocal microscopy , confocal , fluorescence microscope , polarization (electrochemistry) , mitosis , orthogonal polarization spectral imaging , fluorescence lifetime imaging microscopy , materials science , fluorescence , physics , chemistry , biology , laser , microbiology and biotechnology , scattering
We report how a recently developed polarization imaging technique, implementing micro-wave photonics and referred to as orthogonality-breaking (OB) imaging, can be adapted on a classical confocal fluorescence microscope, and is able to provide informative polarization images from a single scan of the cell sample. For instance, the comparison of the images of various cell lines at different cell-cycle stages obtained by OB polarization microscopy and fluorescence confocal images shows that an endogenous polarimetric contrast arizes with this instrument on compacted chromosomes during cell division.