Open AccessCentimeter-scale wide-field-of-view laser-scanning photoacoustic microscopy for subcutaneous microvasculature in vivo
Author(s) -
Tangyun Liao,
Yuan Liu,
JyhHorng Wu,
Lijun Deng,
Yu Deng,
Lvming Zeng,
Xuanrong Ji
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.426366
Subject(s) - optics , microscopy , materials science , preclinical imaging , laser , biomedical engineering , in vivo , resolution (logic) , field of view , laser scanning , light sheet fluorescence microscopy , scanning confocal electron microscopy , medicine , physics , computer science , biology , microbiology and biotechnology , artificial intelligence
We developed a simple and compact laser-scanning photoacoustic microscopy (PAM) for imaging large areas of subcutaneous microvasculature in vivo. The reflection-mode PAM not only retains the advantage of high scanning speed for optical scanning, but also offers an imaging field-of-view (FOV) up to 20 × 20 mm 2 , which is the largest FOV available in laser-scanning models so far. The lateral resolution of the PAM system was measured to be 17.5 µm. Image experiments on subcutaneous microvasculature in in vivo mouse ears and abdomen demonstrate the system's potential for fast and high-resolution imaging for injuries and diseases of large tissues and organs.