Open AccessFull circumferential morphological analysis of Schlemm’s canal in human eyes using megahertz swept source OCT
Author(s) -
Xinwen Yao,
Bingyao Tan,
Yijie Ho,
Xinyu Liu,
Damon Wing Kee Wong,
Jacqueline Chua,
Tina Wong,
Shamira Perera,
Marcus Ang,
René M. Werkmeister,
Leopold Schmetterer
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.426218
Subject(s) - image stitching , segmentation , optical coherence tomography , optics , raster scan , schlemm's canal , fourier domain , computer science , standard deviation , materials science , artificial intelligence , physics , glaucoma , mathematics , ophthalmology , medicine , trabecular meshwork , statistics
We performed full circumferential imaging of the Schlemm's canal (SC) of two human eyes using a Fourier domain mode-lock laser (FDML) based 1.66-MHz SS-OCT prototype at 1060 nm. Eight volumes with overlapping margins were acquired around the limbal area with customized raster scanning patterns designed to fully cover the SC while minimizing motion artifacts. The SC was segmented from the volumes using a semi-automated active contour segmentation algorithm, whose mean dice similarity coefficient was 0.76 compared to the manual segmentation results. We also reconstructed three-dimensional (3D) renderings of the 360° SC by stitching the segmented SCs from the volumetric datasets. Quantitative metrics of the full circumferential SC were provided, including the mean and standard deviation (SD) of the cross-sectional area (CSA), the maximum CSA, the minimum and maximum SC opening width, and the number of collector channels (CC) stemming from the SC.