Open AccessSelf-contained and modular structured illumination microscope
Author(s) -
Robin Van den Eynde,
Wim Vandenberg,
Siewert Hugelier,
Arno Bouwens,
Johan Hofkens,
Marcel Müller,
Peter Dedecker
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.423492
Subject(s) - modular design , schematic , computer science , microscope , software , fluorescence microscope , optics , computer graphics (images) , microscopy , computer hardware , computer vision , artificial intelligence , fluorescence , physics , programming language , engineering , electronic engineering
We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our strategy, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments.