Self-contained and modular structured illumination microscope | Zendy

Robin Van den Eynde | Zendy; Wim Vandenberg | Zendy; Siewert Hugelier | Zendy; Arno Bouwens | Zendy; Johan Hofkens | Zendy; Marcel Müller | Zendy; Peter Dedecker | Zendy

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Self-contained and modular structured illumination microscope

Author(s) -

Robin Van den Eynde,

Wim Vandenberg,

Siewert Hugelier,

Arno Bouwens,

Johan Hofkens,

Marcel Müller,

Peter Dedecker

Publication year - 2021

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.423492

Subject(s) - modular design , schematic , computer science , microscope , software , fluorescence microscope , computer graphics (images) , optics , microscopy , computer hardware , computer vision , artificial intelligence , fluorescence , physics , programming language , engineering , electronic engineering

We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our strategy, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments.

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