Open AccessRaman spectroscopy for guidance of vulvar cancer surgery: a pilot study
Author(s) -
Helena C. van Doorn,
Elisa M. Barroso,
Senada Koljenović,
Patricia C. EwingGraham,
Maria R Nunes Soares,
Nick J. van de Berg,
Tom C. Bakker Schut,
Gerwin J. Puppels
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.420882
Subject(s) - raman spectroscopy , vulvar cancer , cancer , medicine , ex vivo , materials science , biomedical engineering , vulva , pathology , in vivo , optics , biology , physics , microbiology and biotechnology
For vulvar squamous cell carcinoma (VSCC), the mainstay of treatment is surgical removal with tumour-free margins. Surgeons still operate without objective tools that provide margin-status. This study assesses Raman spectroscopy potentiality for distinguishing ex-vivo VSCC from healthy tissue in 11 patients. Grid-based Raman maps were obtained from processed spectra. Water content and C-H band ratio (2,910-2,966 cm -1 / 2810-2890 cm -1 ) were calculated per spectrum and used as linear discriminant parameters. Healthy tissue was differentiated from VSCC with 0.90 discriminative power, 0.79 sensitivity and 0.86 specificity.This is an important step towards the development of objective tools for VSCC surgical guidance.