Open AccessCombination of pathological and spectroscopic characterization to promote diagnosis of retinal pigment epithelium-Bruch’s membrane complex in a diabetic rat model
Author(s) -
Yang Chen,
Ting Xie,
Minlu Ye,
Qiaoling Lai,
Yuting Wang,
Xu Yu,
Wenyi Chen,
Weidong Zheng,
Shouhua Feng,
Yan Huang
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.419716
Subject(s) - diabetic retinopathy , retinal pigment epithelium , bruch's membrane , diabetes mellitus , retinal , glycation , raman spectroscopy , retina , medicine , pathology , endocrinology , chemistry , ophthalmology , biology , neuroscience , optics , physics
Diabetic retinopathy (DR) is a common condition of diabetes, and approaches to detecting early DR using the unique characteristics of the retinal pigment epithelium-Bruch's membrane complex (RBC) have increasingly attracted attention. A diabetic model was established in Sprague-Dawley rats via streptozocin (STZ) injection for 1 (DM1) and 6 months (DM6), confirmed by weekly blood glucose measurement. Serum and retinal tissue-based advanced glycation endproducts (AGE) levels significantly elevated in diabetic rats, and RBC was evaluated by transmission electron microscopy and Raman spectroscopy. The results showed that whole Raman spectra and all marked band intensities could respectively achieve almost equal and accurate discrimination of all animal groups, along with the determination of important molecules from the band data. Further quantitative analyses indicated series of metabolic disturbance due to hyperglycemia were involved while the body self-regulation mechanism still played a role with different effects during the disease progression. Given this, Raman spectroscopy can reliably distinguish the early characterization of DR in addition to providing intrinsic key molecules that is sensitive to identify the early disease progression.