Open AccessDisplacement and strain mapping for osteocytes under fluid shear stress using digital holographic microscopy and digital image correlation
Author(s) -
Runyu Cao,
Wen Xiao,
Feng Pan,
Ran Tian,
Xin-Tong Wu,
Lianwen Sun
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.418418
Subject(s) - digital image correlation , speckle pattern , digital holographic microscopy , materials science , displacement (psychology) , holography , shear stress , digital holography , mechanotransduction , optics , shear (geology) , deformation (meteorology) , physics , composite material , psychology , neuroscience , psychotherapist , biology
Osteocytes, as the mechano-sensors in bone, are always subjected to fluid shear stress (FSS) from the surrounding matrix. Quantification of FSS-induced cellular deformation is significant for clarifying the "perceive and transmit" process of cellular mechanotransduction. In this research, a label-free displacement and strain mapping method based on digital holographic microscopy (DHM) and digital image correlation (DIC) is introduced. The method, which is termed DHM-DIC, innovatively utilizes surface features extracted from holographic phase images instead of speckles as the metric for DIC searching. Simulation results on a hemisphere validate the feasibility of DHM-DIC. Displacement and strain maps of living osteocytes under 1.5 Pa FSS are evaluated from DHM-DIC and present good agreement with our previous finite element modeling results.