Open AccessDual-view light-sheet imaging through a tilted glass interface using a deformable mirror
Author(s) -
Nikita Vladimirov,
Friedrich Preußer,
Jan Wiśniewski,
Ziv Yaniv,
Ravi A. Desai,
Andrew Woehler,
Stephan Preibisch
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.416737
Subject(s) - optics , point spread function , light sheet fluorescence microscopy , microscopy , adaptive optics , deformable mirror , microfluidics , materials science , microscope , computer science , physics , nanotechnology , scanning confocal electron microscopy
Light-sheet microscopy has become indispensable for imaging developing organisms, and imaging from multiple directions (views) is essential to improve its spatial resolution. We combine multi-view light-sheet microscopy with microfluidics using adaptive optics (deformable mirror) which corrects aberrations introduced by the 45 o -tilted glass coverslip. The optimal shape of the deformable mirror is computed by an iterative algorithm that optimizes the point-spread function in two orthogonal views. Simultaneous correction in two optical arms is achieved via a knife-edge mirror that splits the excitation path and combines the detection paths. Our design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experiments and high-content screening.