Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror | Zendy

Nikita Vladimirov | Zendy; Friedrich Preußer | Zendy; Jan Wiśniewski | Zendy; Ziv Yaniv | Zendy; Ravi A. Desai | Zendy; Andrew Woehler | Zendy; Stephan Preibisch | Zendy

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Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror

Author(s) -

Nikita Vladimirov,

Friedrich Preußer,

Jan Wiśniewski,

Ziv Yaniv,

Ravi A. Desai,

Andrew Woehler,

Stephan Preibisch

Publication year - 2021

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.416737

Subject(s) - optics , point spread function , adaptive optics , light sheet fluorescence microscopy , microscopy , deformable mirror , microfluidics , materials science , microscope , computer science , computer vision , physics , nanotechnology , scanning confocal electron microscopy

Light-sheet microscopy has become indispensable for imaging developing organisms, and imaging from multiple directions (views) is essential to improve its spatial resolution. We combine multi-view light-sheet microscopy with microfluidics using adaptive optics (deformable mirror) which corrects aberrations introduced by the 45 o -tilted glass coverslip. The optimal shape of the deformable mirror is computed by an iterative algorithm that optimizes the point-spread function in two orthogonal views. Simultaneous correction in two optical arms is achieved via a knife-edge mirror that splits the excitation path and combines the detection paths. Our design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experiments and high-content screening.

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