Open AccessOptical biopsy of laryngeal lesions using femtosecond multiphoton microscopy
Author(s) -
Hong Zhang,
Yan Chen,
Dingfang Cao,
Wenjing Li,
Yanlei Jing,
Hua Zhong,
Honggang Liu,
Xiangwei Zhu
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.414931
Subject(s) - ex vivo , pathology , in vivo , microscopy , grading (engineering) , multiphoton fluorescence microscope , femtosecond , larynx , optical coherence tomography , biopsy , medicine , malignancy , laryngeal neoplasm , carcinoma , radiology , laser , optics , biology , fluorescence microscope , anatomy , ecology , physics , microbiology and biotechnology , fluorescence
Laryngeal squamous cell carcinoma (LSCC) is one of the most prevalent malignancy of the upper aerodigestive tract. Detection of early lesions in vivo could improve the survival rate significantly. In this study, we demonstrated that femtosecond multiphoton microscopy (MPM) is an effective tool to visualize the microscopic features within fixed laryngeal tissues, without sectioning, staining, or labeling. Accurate detection of lesions and determination of the tumor grading can be achieved, with excellent consistency with conventional histological examination. These results suggest that MPM may represent a powerful tool for in-vivo or fast ex-vivo diagnosis of laryngeal lesions at the point of care.