Open AccessPulsed laser activated impulse response encoder (PLAIRE): sensitive evaluation of surface cellular stiffness on zebrafish embryos
Author(s) -
Ryohei Yasukuni,
Daiki Minamino,
Takanori Iino,
Takashi Araki,
Kohei Takao,
Sohei Yamada,
Yasumasa Bessho,
Takaaki Matsui,
Yoichiroh Hosokawa
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.414338
Subject(s) - femtosecond , cantilever , stiffness , materials science , impulse (physics) , laser , vibration , cytoskeleton , atomic force microscopy , excited state , optics , biophysics , nanotechnology , acoustics , chemistry , physics , composite material , biology , biochemistry , quantum mechanics , cell , nuclear physics
Mechanical properties of cells and tissues closely link to their architectures and physiological functions. To obtain the mechanical information of submillimeter scale small biological objects, we recently focused on the object vibration responses when excited by a femtosecond laser-induced impulsive force. These responses are monitored by the motion of an AFM cantilever placed on top of a sample. In this paper, we examined the surface cellular stiffness of zebrafish embryos based on excited vibration forms in different cytoskeletal states. The vibration responses were more sensitive to their surface cellular stiffness in comparison to the Young's modulus obtained by a conventional AFM force curve measurement.