Open AccessFluorescent bacterial biosensor E. coli/pTdcR-TurboYFP sensitive to terahertz radiation
Author(s) -
Danil S. Serdyukov,
T. N. Goryachkovskaya,
Irina Anatolyevna Mescheryakova,
Sergei A. Kuznetsov,
V. M. Popik,
Sergey E. Peltek
Publication year - 2021
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.412074
Subject(s) - fluorescence , biosensor , escherichia coli , terahertz radiation , green fluorescent protein , biophysics , chemistry , biochemistry , biology , materials science , optoelectronics , optics , physics , gene
A fluorescent biosensor E. coli /pTdcR-TurboYFP sensitive to terahertz (THz) radiation was developed via transformation of Escherichia coli ( E. coli ) cells with plasmid, in which the promotor of the tdcR gene controls the expression of yellow fluorescent protein TurboYFP. The biosensor was exposed to THz radiation in various vessels and nutrient media. The threshold and dynamics of fluorescence were found to depend on irradiation conditions. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensor is applicable to studying influence of THz radiation on the activity of tdcR promotor that is involved in the transport and metabolism of threonine and serine in E. coli .