Open AccessDual modal fluorescent colposcope combined with near-infrared fluorescent dye TMTP1-PEG4-ICG to detect cervical lesions
Author(s) -
Chen Wang,
Ying Zhou,
Wei Li,
Liu Xiao-hu,
Xi Liu,
Pengcheng Li,
Juncheng Wei,
Jinling Lu
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.410394
Subject(s) - fluorescence , cervical intraepithelial neoplasia , materials science , fluorescence lifetime imaging microscopy , indocyanine green , optics , medicine , pathology , cervical cancer , cancer , physics
To examine the cervical lesions by using the tumor-targeted near-infrared (NIR) fluorescent dyes TMTP1-PEG4-ICG, a dual modal colposcope with visible reflectance imaging and fluorescence imaging was developed. NIR fluorescence imaging and visible light reflectance imaging were integrated together on a colposcope by designing the specific optics and adopting a dual sensor charge coupled device (CCD) camera. Patients with squamous cell carcinoma, adenocarcinoma, cervical intraepithelial neoplasia (CIN) and cervicitis were examined using this dual modal colposcope to validate its potential of cervical cancer detection. Fluorescent dye TMTP1-PEG4-ICG was applied to the cervix 30 minutes before inspection. The fluorescence images were collected after wiping the unbound fluorescent dye using normal saline. Signal to back ratios (SBR) of the fluorescence images were analyzed and compared with the histological analysis. The results suggest that the fluorescent colposcope combined with tumor-specific near-infrared fluorescent dyes TMTP1-PEG4-ICG could help to evaluate cervical lesions in real time.