Open AccessMulti-resolution open-top light-sheet microscopy to enable efficient 3D pathology workflows
Author(s) -
Lindsey A. Barner,
Adam K. Glaser,
Hongyi Huang,
Lawrence D. True,
Jonathan Liu
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.408684
Subject(s) - microscopy , light sheet fluorescence microscopy , workflow , microscope , computer science , lens (geology) , virtual microscopy , image resolution , optics , materials science , computer vision , scanning confocal electron microscopy , physics , database
Open-top light-sheet (OTLS) microscopes have been developed for user-friendly and versatile high-throughput 3D microscopy of thick specimens. As with all imaging modalities, spatial resolution trades off with imaging and analysis times. A hierarchical multi-scale imaging workflow would therefore be of value for many volumetric microscopy applications. We describe a compact multi-resolution OTLS microscope, enabled by a novel solid immersion meniscus lens (SIMlens), which allows users to rapidly transition between air-based objectives for low- and high-resolution 3D imaging. We demonstrate the utility of this system by showcasing an efficient 3D analysis workflow for a diagnostic pathology application.