Open AccessDetection of singlet oxygen luminescence for experimental corneal rose bengal photodynamic antimicrobial therapy
Author(s) -
Jeffrey Peterson,
Esdras Arrieta,
Marco Ruggeri,
Juan Silgado,
Keenan J. Mintz,
Ernesto H. Weisson,
Roger M. Leblanc,
Irene E. Kochevar,
Fabrice Manns,
Jean-Marie A. Parel
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.405601
Subject(s) - rose bengal , singlet oxygen , photodynamic therapy , luminescence , photosensitizer , cornea , dosimeter , antimicrobial , materials science , dosimetry , irradiation , optics , photochemistry , optoelectronics , oxygen , chemistry , medicine , nuclear medicine , physics , organic chemistry , nuclear physics
Rose bengal photodynamic antimicrobial therapy (RB-PDAT) treats corneal infection by activating rose bengal (RB) with green light to produce singlet oxygen ( 1 O 2 ). Singlet oxygen dosimetry can help optimize treatment parameters. We present a 1 O 2 dosimeter for detection of 1 O 2 generated during experimental RB-PDAT. The system uses a 520 nm laser and an InGaAs photoreceiver with bandpass filters to detect 1 O 2 luminescence during irradiation. The system was validated in RB solutions and ex vivo in human donor eyes. The results demonstrate the feasibility of 1 O 2 dosimetry in an experimental model of RB-PDAT in the cornea.