Open AccessThree-dimensional label-free imaging and quantification of migrating cells during wound healing
Author(s) -
Ariel J. Lee,
Hervé Hugonnet,
Weisun Park,
YongKeun Park
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.405087
Subject(s) - wound healing , live cell imaging , optical imaging , dynamics (music) , biomedical engineering , molecular imaging , biophysics , nanotechnology , cell , chemistry , materials science , optics , medicine , biology , in vivo , physics , surgery , biochemistry , microbiology and biotechnology , acoustics
The wound-healing assay is a simple but effective tool for studying collective cell migration (CCM) that is widely used in biophysical studies and high-throughput screening. However, conventional imaging and analysis methods only address two-dimensional (2D) properties in a wound healing assay, such as gap closure rate. This is unfortunate because biological cells are complex 3D structures, and their dynamics provide significant information about cell physiology. Here, we presented 3D label-free imaging for wound healing assays and investigated the 3D dynamics of CCM using optical diffraction tomography. High-resolution subcellular structures as well as their collective dynamics were imaged and analyzed quantitatively.