Open AccessApplication of quantitative second-harmonic generation microscopy to dynamic conditions
Author(s) -
Mohammad M. Kabir,
V. V. G. Krishna Inavalli,
Tung Yuen Lau,
Kimani C. Toussaint
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.4.002546
Subject(s) - optics , second harmonic generation , microscopy , collagen fiber , pixel , sample (material) , materials science , image processing , signal to noise ratio (imaging) , signal (programming language) , image resolution , computer science , image (mathematics) , biological system , artificial intelligence , physics , laser , medicine , anatomy , programming language , thermodynamics , biology
We present a quantitative second-harmonic generation (SHG) imaging technique that quantifies the 2D spatial organization of collagen fiber samples under dynamic conditions, as an image is acquired. The technique is demonstrated for both a well-aligned tendon sample and a randomly aligned, sparsely distributed collagen scaffold sample. For a fixed signal-to-noise ratio, we confirm the applicability of this method for various window sizes (pixel areas) as well as with using a gridded overlay map that allows for correlations of fiber orientations within a given image. This work has direct impact to in vivo biological studies by incorporating simultaneous SHG image acquisition and analysis.