Open AccessSignal and depth enhancement for in vivo flow cytometer measurement of ear skin by optical clearing agents
Author(s) -
Yimin Ding,
Jing Wang,
Zhichao Fan,
Dan Wei,
Rui Shi,
Qingming Luo,
Dan Zhu,
Xunbin Wei
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.4.002518
Subject(s) - in vivo , signal (programming language) , biomedical engineering , flow cytometry , light scattering , pathology , materials science , optics , medicine , scattering , computer science , biology , physics , immunology , microbiology and biotechnology , programming language
The in vivo flow cytometry (IVFC) has shown a great potential for detecting circulating tumor cells quantitatively in the bloodstream. However, the detection depth suffers from the strong light scattering of tissue. In this study, an innovative ear skin optical clearing agent (ESOCA) is employed to improve the signal quality of the IVFC. Our results show that compared with commonly used glycerol, topical application of ESOCA can enhance the transmittance of rat ear significantly in vivo. The labeled red blood cells can be detected by the IVFC with higher signal quality and greater detection depth. This study is very helpful for potential tumor metastasis studies by the IVFC in deep tissues.