Open AccessTri-modal microscopy with multiphoton and optical coherence microscopy/tomography for multi-scale and multi-contrast imaging
Author(s) -
Shau Poh Chong,
Tom Lai,
Yifeng Zhou,
Shuo Tang
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.4.001584
Subject(s) - microscopy , optical coherence tomography , optics , microscope , fluorescence microscope , two photon excitation microscopy , materials science , fluorescence lifetime imaging microscopy , physics , fluorescence
Multi-scale multimodal microscopy is a very useful technique by providing multiple imaging contrasts with adjustable field of views and spatial resolutions. Here, we present a tri-modal microscope combining multiphoton microscopy (MPM), optical coherence microscopy (OCM) and optical coherence tomography (OCT) for subsurface visualization of biological tissues. The advantages of the tri-modal system are demonstrated on various biological samples. It enables the visualization of multiple intrinsic contrasts including scattering, two-photon excitation fluorescence (TPEF), and second harmonic generation (SHG). It also enables a rapid scanning over a large tissue area and a high resolution zoom-in for cellular-level structures on regions of interest. The tri-modal microscope can be important for label-free imaging to obtain a sufficient set of parameters for reliable sample analysis.