Simultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy | Zendy

Keisuke Isobe | Zendy; Hiroyuki Kawano | Zendy; Akira Suda | Zendy; Akiko Kumagai | Zendy; Atsushi Miyawaki | Zendy; Katsumi Midorikawa | Zendy

Open Access

Simultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy

Author(s) -

Keisuke Isobe,

Hiroyuki Kawano,

Akira Suda,

Akiko Kumagai,

Atsushi Miyawaki,

Katsumi Midorikawa

Publication year - 2013

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.4.001548

Subject(s) - raman scattering , absorption (acoustics) , two photon absorption , raman spectroscopy , optics , materials science , x ray raman scattering , microscopy , scattering , photon , nonlinear optics , quantum dot , molecular physics , optoelectronics , physics , laser

Imaging of simultaneous two-photon absorption and stimulated Raman scattering is accomplished by detecting the intensity changes of the two-color pulses simultaneously and the mathematical operations of addition and subtraction. The stimulated Raman scattering is quantitatively separated from the two-photon absorption, generated in a mixed solution in which a glycerin solution is miscible in various proportions with a quantum dot solution. Our technique is applied to simultaneous two-photon absorption and stimulated Raman scattering imaging.

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