Open AccessSimultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy
Author(s) -
Keisuke Isobe,
Hiroyuki Kawano,
Akira Suda,
Akiko Kumagai,
Atsushi Miyawaki,
Katsumi Midorikawa
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.4.001548
Subject(s) - raman scattering , absorption (acoustics) , two photon absorption , raman spectroscopy , optics , materials science , x ray raman scattering , microscopy , scattering , photon , nonlinear optics , quantum dot , molecular physics , optoelectronics , physics , laser
Imaging of simultaneous two-photon absorption and stimulated Raman scattering is accomplished by detecting the intensity changes of the two-color pulses simultaneously and the mathematical operations of addition and subtraction. The stimulated Raman scattering is quantitatively separated from the two-photon absorption, generated in a mixed solution in which a glycerin solution is miscible in various proportions with a quantum dot solution. Our technique is applied to simultaneous two-photon absorption and stimulated Raman scattering imaging.