Open AccessIn vitro measurements of physiological glucose concentrations in biological fluids using mid-infrared light
Author(s) -
Sabbir Liakat,
Kevin A. Bors,
Tzu-Yung Huang,
Anna P. M. Michel,
Eric Zanghi,
Claire F. Gmachl
Publication year - 2013
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.4.001083
Subject(s) - quantum cascade laser , infrared , infrared spectroscopy , partial least squares regression , spectroscopy , calibration , materials science , in vivo , chemistry , analytical chemistry (journal) , optics , cascade , chromatography , biology , mathematics , physics , quantum mechanics , statistics , microbiology and biotechnology , organic chemistry
Mid-infrared transmission spectroscopy using broadband mid-infrared or Quantum Cascade laser sources is used to predict glucose concentrations of aqueous and serum solutions containing physiologically relevant amounts of glucose (50-400 mg/dL). We employ partial least squares regression to generate a calibration model using a subset of the spectra taken and to predict concentrations from new spectra. Clinically accurate measurements with respect to a Clarke error grid were made for concentrations as low as 30 mg/dL, regardless of background solvent. These results are an important and encouraging step in the work towards developing a noninvasive in vivo glucose sensor in the mid-infrared.