Open AccessFunctional optical coherence tomography enables in vivo optoretinography of photoreceptor dysfunction due to retinal degeneration
Author(s) -
Tae-Hoon Kim,
Benquan Wang,
Yiming Lu,
Taeyoon Son,
Xincheng Yao
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.399334
Subject(s) - visual phototransduction , optical coherence tomography , retinal , retinal degeneration , retina , electroretinography , retinal regeneration , in vivo , optics , ophthalmology , biology , medicine , physics , microbiology and biotechnology
Stimulus-evoked intrinsic optical signal (IOS), which occurs almost immediately after the onset of retinal stimulus has been observed in retinal photoreceptors, promises to be a unique biomarker for objective optoretinography (ORG) of photoreceptor function. We report here the first-time in vivo ORG detection of photoreceptor dysfunction due to retinal degeneration. A custom-designed optical coherence tomography (OCT) was employed for longitudinal ORG monitoring of photoreceptor-IOS distortions in retinal degeneration mice. Depth-resolved OCT analysis confirmed the outer segment (OS) as the physical source of the photoreceptor-IOS. Comparative ERG measurement verified the phototransduction activation as the physiological correlator of the photoreceptor-IOS. Histological examination revealed disorganized OS discs, i.e. the pathological origin of the photoreceptor-IOS distortion.