Spatial scanning of a sample with two-dimensional angle-resolved low-coherence interferometry for analysis of anisotropic scatterers | Zendy

Ge Song | Zendy; Zachary A. Steelman | Zendy; Wesley Y. Kendall | Zendy; Han Sang Park | Zendy; Adam Wax | Zendy

Open Access

Spatial scanning of a sample with two-dimensional angle-resolved low-coherence interferometry for analysis of anisotropic scatterers

Author(s) -

Ge Song,

Zachary A. Steelman,

Wesley Y. Kendall,

Han Sang Park,

Adam Wax

Publication year - 2020

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.398052

Subject(s) - optics , interferometry , scattering , coherence (philosophical gambling strategy) , optical coherence tomography , anisotropy , spatial frequency , materials science , rotation (mathematics) , sample (material) , computation , physics , computer science , artificial intelligence , algorithm , quantum mechanics , thermodynamics

Angle-resolved low-coherence interferometry (a/LCI) measures depth-resolved angular scattering for cell nuclear morphology analysis. 2D a/LCI, developed to collect across two scattering planes, is currently limited by the lack of spatial scanning. Here we demonstrate 2D a/LCI scanning across a three-dimensional volume using an image rotation scheme and a scanning mirror. Validation using various optical phantoms demonstrated excellent scatterer size determination over a 7.5 mm linear range, for a total accessible area of ∼44 mm 2 . Measurements from anisotropic scatterers allowed accurate determination of sizes and computation of aspect ratios. This scanning system will facilitate analysis of scatterer structure across wider tissue areas.

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