Open AccessIrradiation effect of a submillimeter wave from 420 GHz gyrotron on amyloid peptides in vitro
Author(s) -
Takayasu Kawasaki,
Y. Yamaguchi,
Tomomi Ueda,
Yuko Ishikawa,
Toyonari Yaji,
Toshiaki Ohta,
Koichi Tsukiyama,
T. Idehara,
Masatoshi Saiki,
Masahiko Tani
Publication year - 2020
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.395218
Subject(s) - irradiation , infrared , gyrotron , synchrotron radiation , amyloid (mycology) , materials science , optics , fluorescence , radiation , microscopy , biophysics , chemistry , analytical chemistry (journal) , electron , physics , biology , chromatography , inorganic chemistry , quantum mechanics , nuclear physics
On using the far-infrared radiation system, whether the irradiation effect is thermal or non-thermal is controversial. We irradiated amyloid peptides that are causal factors for amyloidosis by using a submillimeter wave from 420 GHz gyrotron. Fluorescence reagent assay, optical and electron microscopies, and synchrotron-radiation infrared microscopy showed that the irradiation increased the fibrous conformation of peptides at room temperature for 30 min. The temperature increase on the sample was only below 5 K, and a simple heating up to 318 K hardly induced the fibril formation. Therefore, the amyloid aggregation was driven by the far-infrared radiation with little thermal effect.